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Loading Control Antibodies

Loading control antibodies are used to determine equal loading as well as the quality of the samples. This is critically important to aid in the interpretation of western blot results. Proteins used as loading controls are typically selected for their ubiquitous expression across all cell lines and tissues as well as their abundance. Selecting the right loading control antibody is determined by the molecular weight of the other targets being studied, subcellular fraction of the sample, and the cell and tissue types being evaluated.

You need to trust that your loading control is sensitive and specific to its target protein. If your loading control is questionable, it can create doubt about all of your results. Use loading controls you trust for the most reliable experimental results.

Loading Control Antibodies

Loading Control Size (kDa) Recommended for:
β-Actin 45 Whole Cell Lysate
GAPDH 37
β-Tubulin 55
α-Tubulin 52
Vinculin 124
α-Actinin 100
Cyclophilin B 19
Loading Control Size (kDa) Recommended for:
Histone H3 17 Nuclear Fraction
HDAC1 62
Loading Control Size (kDa) Recommended for:
COX IV 17 Mitochondrial Fraction
VDAC 32
SDHA 70

Loading Control Antibody Sampler Kits

No. Name
5142 Loading Control Antibody Sampler Kit
9774 Loading Control Antibody Sampler Kit (Mouse)
4670 Loading Control Antibody Sampler Kit (HRP Conjugate)