Cat. # | Size | Qty. | Price |
---|---|---|---|
13079S | 1 Kit (120 slides) |
|
Product Includes | Quantity | ||||
---|---|---|---|---|---|
SignalStain® DAB Substrate Kit 8059 | 1 Kit | ||||
SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) 8114 | 12 ml | ||||
SignalStain® Antibody Diluent 8112 | 25 ml | ||||
Animal-Free Blocking Solution (5X) 15019 | 4 ml |
Product Information
NOTE: Please refer to the primary antibody product webpage for recommended primary antibody dilution buffer and recommended antibody dilution.
SUPPLIED REAGENTS
1.0 ml of 1X Blocking Solution, add 200 μl of Animal-Free blocking solution and 800 μl of dH2O just before use. Always prepare fresh 1X solutions daily.
ADDITIONAL REAGENTS (NOT SUPPLIED)
NOTE: Consult product datasheet for specific unmasking solution recommendation.
NOTE: Do not allow slides to dry at any time during this procedure.
NOTE: Consult product datasheet for specific unmasking solution recommendation.
NOTE: Consult product datasheet for specific dilution recommendations for each primary antibody.
posted June 2020
Protocol Id: 2105
The Immunohistochemistry Application Solutions Kit (IHC-P) is designed to conveniently provide supporting reagents needed for immunohistochemistry staining in paraffin-embedded tissue samples or cell pellets (IHC-P). The reagents in this kit are thoroughly validated using our IHC-recommended rabbit polyclonal and monoclonal antibodies and will perform optimally with the CST immunohistochemistry staining protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents for 120 slides based on a 100 µl assay volume. All reagents in this kit are available individually.
IMPORTANT: Please refer to the primary antibody datasheet to determine if the antibody is approved for use on paraffin-embedded samples (IHC-P) and for information regarding the appropriate antibody dilution, diluent, and antigen unmasking procedure.
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