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Kinase Buffer (10X)
Buffers & Dyes

Kinase Buffer (10X) #9802

Kinase Buffer can be used to assay protein kinase activity.

1. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For longer periods of time, buffer should be stored at –20°C. Aliquotting of 10X buffer is recommended if many small experiments are to be performed.

2. Thaw 10X buffer at 24-30°C, mixing end-over-end.

3. Dilute the 10X buffer to the desired concentration using ddH2O.

Solutions and Reagents:

1X Kinase Buffer

25 mM Tris-HCl (pH 7.5), 5 mM beta-glycerophosphate, 2 mM dithiothreitol (DTT), 0.1 mM Na3VO4, 10 mM MgCl2.

Note: Kinase Buffer must be supplemented with ATP (#9804).


Store at -20C. For short term storage (1-2 weeks), Kinase Buffer can be stored at 4°C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 9802S

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Product # Size Price
15 ml N/A

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