After dehydration and clearing of samples on a slide, apply a small amount of SignalStain® Mounting Medium either on or near the section using a pipette. When the coverslip is applied, the medium will spread evenly across the coverslipped area.
Please allow the preparation to dry completely. Drying may take several hours when left on the lab bench, however this process can be accelerated by incubating the slides for 15 – 30 minutes at 60ºC to 75ºC. Please ensure the slides have cooled before storage.
SignalStain® Mounting Medium should be stored at room temperature and is stable for 12 months. Keep container tightly sealed when not in use. Slight cloudiness may appear, however this will have no adverse effects.
SignalStain® Mounting Medium is for use in immunohistochemical assays. This product permanently preserves tissue or cell preparations, and produces clear mounted sections, free from crystallization, with the ideal refractive index for high-resolution oil immersion microscopy. SignalStain® Mounting Medium retains the staining intensity of slides prepared with SignalStain® DAB Substrate Kit #8059.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.