Acetyl-CoA Carboxylase Blocking Peptide #1062

This peptide is used to block Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 or Acetyl-CoA Carboxylase Antibody #3662 reactivity by dot blot and Western blot.

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 and Acetyl-CoA Carboxylase Antibody #3662 by dot blot and Western blot.

Use as a blocking reagent to evaluate the specificity of antibody reactivity in dot blot protocols.

Storage:

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

Data sheet (84KB)