Buy 3 Get a 4th Free* | Learn More >>
7780
BCA Protein Assay Kit

BCA Protein Assay Kit #7780

 Image 1

Standard curves generated with bovine serum albumin (BSA) ranging from 125 to 2,000 μg/ml using Cell Lysis Buffer #9803, RIPA Buffer #9806, and PathScan® Sandwich ELISA Lysis Buffer #7018.

Learn more about how we get our images
Product Includes Quantity
BCA Protein Assay Reagent A 250 ml
BCA Protein Assay Reagent B 25 ml
BCA Protein Assay Albumin Standard 10 units
Compatibility Reagent 48 ea
Reconstitution Buffer 15 ml

The BCA Protein Assay Kit can be used to measure the protein concentration of lysates or homogenates, in microplate format, prepared with the following buffers: Cell Lysis Buffer (10X) #9803, RIPA Buffer (10X) #9806, PathScan® Sandwich ELISA Lysis Buffer (1X) #7018. The dynamic range for this assay is 0.125 - 2 mg/mL. It is recommended that the BCA Compatibility Reagent be used to decrease interference from reducing agents, chelators, detergents, and other common ingredients found in most lysis buffers. Please see the attached protocol for additional details.

Storage:

Store at room temperature.

Bicinchoninic Acid (BCA) is capable of forming an intense purple complex with cuprous ion, Cu1+, in an alkaline environment. Cu1h+ is produced from the reaction of protein with alkaline Cu2+. The resulting reaction and color produced is the basis for a common protein quantification method capable of measuring protein concentration over a wide range. Increasing protein concentrations produce proportionally deeper colors. The BCA protein assay demonstrates higher tolerances towards common interfering substances, such as nonionic detergents and buffer salts, than the Lowry technique (1).

  1. Smith, P.K. et al. (1985) Anal Biochem 150, 76-85.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

To Purchase # 7780S

Product Number Size
7780S 1 Kit (960 assays)

Your Local Representative for Austria

New England Biolabs GmbH

Bruningstrasse 50, Geb. G 810

65926 Frankfurt am Main

Phone:
+49/ (0) 69 305 23140
Fax:
+49/ (0) 69 305 23149
Phone:
0800/246 5227 [Toll Free]
Fax:
0800/246 5229 [Toll Free]
Email:
info@de.neb.com
URL:
www.neb-online.de

Need information for a different country? Please click here.

To get local purchase information on this product, click here.